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Distribution and histological localization of six types of enzymes in the digestive mucosa of Rana nigromaculata

Liu Ying;Lu Xuerui;Jia Nannan;Huang Yi;Liu Xiaojuan;Zhang Shengzhou;Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province,College of Life Sciences,Anhui Normal University;  
Objective:To investigate the distribution and histological localization of acid phosphatase(ACP),alkaline phosphatase(ALP),nonspecific esterase(NSE),peroxidase(POX),succinate dehydrogenase(SDH),and adenosine triphosphatase(ATPase) in the digestive tract of Rana nigromaculata.Methods:Tissue samples from adult Rana nigromaculata were obtained from eight parts of the digestive tract from the esophagus to the rectum and studied by frozen section,enzymatic histochemical techniques and quantitative analysis of optical density.Results:ACP was present throughout the digestive tract and mainly distributed in the top of the enterocytes and gastric glands,with the highest activity in the jejunum;ALP was distributed in the mucosal epithelium of the intestine from duodenum to rectum,with the highest enzymatic activity in duodenum and ileum;NSE,POX and SDH were all detected in the digestive mucosa from esophagus to rectum.NSE was located in the epithelium of the digestive mucosa and gastric glands,with the highest activity in stomachus cardiacus,stomachus corpus and duodenum;POX was mainly concentrated in gastric glands,the muscularis mucosa and lamina propria in the small intestine,with the highest activity in the small intestine;SDH existed in the mucosal epithelium of the digestive tract and gastric glands,with the highest activity in stomachus cardiacus,stomachus corpus and jejunum;ATPase was detected in the entire digestive tract except for rectum and mainly distributed in the brush border of the mucosal epithelium in the small intestine,with the highest activity in duodenum and jejunum.Conclusion:The distribution of enzymes in the digestive mucosa of Rana nigromaculata is similar to that of other animals,but some unique features are also observed,which is adaptive to its special feeding habits and digestive functions.The duodenum,jejunum and ileum are all the main digestive and absorptive sites.
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